Open AccessEnhanced intraspecific protoplast fusion in yeast
Author(s) -
Kavanagh K.,
Walsh M.,
Whittaker P.A.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04773.x
Subject(s) - protoplast , propionate , calcium , polyethylene glycol , yeast , intraspecific competition , peg ratio , biochemistry , candida albicans , cell fusion , saccharomyces cerevisiae , biology , chemistry , biophysics , microbiology and biotechnology , cell , organic chemistry , ecology , finance , economics
Intraspecific hybrid production from the polyethylene glycol induced fusion of yeast protoplasts was greatly increased when calcium propionate was included as the source of the requisite Ca 2+ . The use of calcium propionate, as opposed to the more commonly employed calcium chloride, resulted in substantially greater yields of hybrids from intraspecific fusions of protoplasts of Saccharomyces cerevisiae and Candida albicans . It is postulated that the ability of calcium propionate to enhance the fusion frequency is due to the anion binding to the etheric oxygen of PEG and potentiating the fusogenicity of the polymer.