Open AccessThe proteolytic system of the fission yeast Schizosaccharomyces pombe
Author(s) -
SuárezRendueles Paz,
Villa Lourdes,
Arbesú María José,
Escudero Blanca
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04748.x
Subject(s) - schizosaccharomyces pombe , aminopeptidase , biochemistry , yeast , carboxypeptidase , proteolytic enzymes , enzyme , chemistry , schizosaccharomyces , biology , leucine , amino acid , saccharomyces cerevisiae
Proteinase and peptidase activities of the fission yeast Schizosaccharomyces pombe were investigated. Several intracellular proteolytic enzymes were found: two endoproteinases, one carboxypeptidase, one aminopeptidase and one dipeptidyl‐aminopeptidase. In addition, proteinase inhibitors were detected. In fresh crude extracts an activation procedure is needed to measure maximal activities of endoproteinases and carboxypeptidase, whose level is markedly dependent on growth medium composition and on growth phase, while aminopeptidase and dipeptidyl‐aminopeptidase activities are very little, if at all, regulated by the carbon source.