Open AccessGlycerol and propanediols degradation by Desulfovibrio alcoholovorans in pure culture in the presence of sulfate, or in syntrophic association with Methanospirillum hungatei
Author(s) -
Qatibi A.I.,
Cayol J.L.,
Garcia J.L.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04729.x
Subject(s) - propanediol , glycerol , desulfovibrio , sulfate , 1,3 propanediol , chemistry , nuclear chemistry , biochemistry , biology , organic chemistry
In a mineral medium containing sulfate as terminal electron acceptor, the sulfate‐reducing bacterium Desulfovibrio alcoholovorans oxidized stoichiometrically 1 mol glycerol to 1 mol acetate and 1 mol 1,3‐propanediol to 1 mol acetate with the concomitant reduction of 0.75 and 1 mol sulfate, respectively; 1 mol 1,2‐propanediol was degraded to 0.8 mol acetate and 0.1 mol proprionate, with the reduction of approximately 1 mol sulfate. The maximum specific growth rates (μ max in h −1 ) were 0.22, 0.086 and 0.09 with glycerol, 1,3‐propanediol and 1,2‐propanediol, respectively. The growth yields were 12.7 g, 11.1 g and 7.2 g dry weight/mol 1,3‐propanediol, glycerol and 1,2‐propanediol degraded, respectively. The growth yields and maximum specific growth rates of the H 2 ‐transferring associations were also calculated. In the absense of sulfate, all these reduced substrates were degraded to acids and methane when D. alcoholovorans was cocultured with Methanospirillum hungatei . Changes in the metabolic pathway were observed in the degradation of 1,2‐ and 1,3‐propanediol. The metabolic efficiency of D. alcoholovorans to degrade glycerol, 1.2‐ and 1,3‐propanediol is discussed.