Open AccessPartial characterization of an 89‐kDa highly immunoreactive protein from Chlamydia psittaci A/22 causing ovine abortion
Author(s) -
Cevenini Roberto,
Donati Manuela,
Brocchi Emiliana,
Simone Franco,
Placa Michele
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04722.x
Subject(s) - chlamydia psittaci , abortion , chlamydia , chlamydiaceae , virology , microbiology and biotechnology , biology , chlamydiales , immunology , pregnancy , genetics
An 89‐kDa immunogen from Chlamydia psittaci A/22 causing ovine abortion was partially characterized. The 89‐kDa protein, localized on the outer membrane complex of chlamydiae, was synthesized relatively early in the developmental cycle. The protein contained cysteine but was not extensively cross‐linked by disulfide bonds. Treatment with proteases apparently did not cleave the protein. The infectivity of strain A/22 was partially (60%) reduced by treatment of chlamydial elementary bodies with monoclonal antibody BS/89 specifically reacting with the 89‐kDa antigen. Species‐specific as well as strain‐specific antigenic determinants were present on the 89‐kDa protein.