Open AccessCharacterization of a coproporphyrin‐protein complex from Rhodobacter capsulatus
Author(s) -
Biel Alan J.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04709.x
Subject(s) - rhodobacter , ammonium sulfate , strain (injury) , chemistry , biochemistry , ammonium , sulfate , ammonium sulfate precipitation , chromatography , biology , mutant , size exclusion chromatography , enzyme , organic chemistry , anatomy , gene
Rhodobacter capsulatus strain AJB530 excretes large amounts of coproporphyrin into the culture supernatant. The coproporphyrin was precipitable with ammonium sulfate, suggesting that it was part of a macromolecular complex. Analysis of an ammonium sulfate fraction indicated that the coproporphyrin was bound to a 66‐kDa protein with a p I ′ of 4.0. The same protein was found in the culture supernatant of the bch + strain PAS100.