Open AccessDistribution of the IS elements ISS1 and IS904 in lactococci
Author(s) -
Schäfer A.,
Jahns A.,
Geis A.,
Teuber M.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04681.x
Subject(s) - plasmid , biology , lactococcus lactis , oligonucleotide , gene , microbiology and biotechnology , genetics , bacteria , lactic acid
A broad distribution of the lactococcal IS elements IS S1 [1] and IS 904 [2] in several lactococcal plasmids and chromosomal DNA was observed. Hybridization of the IS S1 and IS 904 oligonucleotide gene probes with DNA of lactococcal phages showed that none of these tested bacteriophages contained one of the IS elements. On the transductionally shortened lactose plasmid pTD1 an insertion sequence homologous to IS S1 was identified closely downstream to the P‐β‐galactosidase gene. Sequence analysis of IS S1 /pTD1 showed 82% homology in the deduced amino acid sequence to the putative transposase of IS S1 , IS S1 W, IS S1 N, and IS 946 .