Open AccessExpression of Staphylococcus aureus enterotoxin type D in Escherichia coli X1776
Author(s) -
Brown Robert C.,
Bingham Alistair H.A.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04679.x
Subject(s) - enterotoxin , escherichia coli , staphylococcus aureus , microbiology and biotechnology , chemistry , biology , bacteria , gene , genetics , biochemistry
The Staphylococcus aureus enterotoxin type D was cloned and expressed in Escherichia coli X1776 either as mature toxin or as a fusion with E. coli β‐galactosidase. Regulated expression was obtained and levels of toxin produced were in the order of 10 −3 ‐fold higher than in S. aureus .