Open AccessIdentification of a telomeric fragment from the right arm of chromosome III of Aspergillus nidulans
Author(s) -
Connelly John C.,
Arst Herbert N.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04678.x
Subject(s) - aspergillus nidulans , fragment (logic) , identification (biology) , biology , genetics , chromosome , aspergillus , long arm , microbiology and biotechnology , computational biology , gene , botany , computer science , mutant , programming language
A minimum of 11 bands hybridising to an oligonucleotide complementary to the putative telomeric repeat sequence (TTAGGG) n was visible in a Southern blot of Eco RI‐digested Aspergillus nidulans genomic DNA. All 11 were sensitive to BAL 31 exonuclease digestion, consistent with telomeric locations. Blots of DNA from aneuploid strains deleted for a dispensable, extreme distal region on the right arm of chromosome III lack a 1.3‐kb Eco RI band, indicating that this fragement is located at or near the chromosome III right arm telomere.