Open AccessCloning and expression of various staphylococcal genes encoding urease in Staphylococcus carnosus
Author(s) -
Jose Joachim,
Christians Stefan,
Rosenstein Ralf,
Götz Friedrich,
Kaltwasser Heinrich
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04675.x
Subject(s) - staphylococcus xylosus , plasmid , recombinant dna , staphylococcus aureus , urease , microbiology and biotechnology , gene , biology , chemistry , cloning (programming) , molecular cloning , staphylococcus , bacteria , enzyme , gene expression , genetics , biochemistry , computer science , programming language
The urease genes from Staphylococcus xylosus C2a, Staphylococcus aureus U500, and S. aureus Newman were cloned in Staphylococcus carnosus using the plasmid vectors pCA43 and pCA44. The resulting respective recombinant plasmids pUra 402, pUraUH66, and pUra17 contained chromosomal DNA fragments with sizes of 5.6, 5.8, and 6.8 kb, respectively. Investigations on urease expression of the donor and recombinant strains in media with various nitrogen sources revealed that S. xylosus C2a produced urease constitutively at the highest specific activity. All of the recombinant strains had significantly lower urease activities than their DNA‐donor strains. The nickel‐dependence of urease was demonstrated in S. aureus U500 by a plate diffusion assay.