Open AccessIdentification, cloning and sequencing of the replication region of Lactococcus lactis ssp. lactis biovar. diacetylactis Bu2 citrate plasmid pSL2
Author(s) -
Jahns A.,
Schäfer A.,
Geis A.,
Teuber M.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04671.x
Subject(s) - plasmid , biovar , lactococcus lactis , replicon , biology , cloning (programming) , bacteria , genetics , origin of replication , microbiology and biotechnology , gene , lactic acid , computer science , programming language
The replication region of the 7.8 kilobase (kb) citrate plasmid pSL2 from Lactococcus lactis ssp. lactis biovar. diacetylactis Bu2 was identified. Deletion derivatives of pSL2 were introduced into plasmid‐free strain Bu2‐60 and tested for their ability to replicate autonomously. The region necessary for replication was identified by comparison of the pSL2 derivatives, cloned and sequenced. No homologies were detected by comparing the putative Rep protein of pSL2 with replicons of other plasmids of Gram‐positive bacteria. A part of an IS‐element flanking the replication region was found.