Open AccessIsolation and characterization of R‐primes of Azotobacter vinelandii
Author(s) -
Blanco G.,
Gutierrez J.C.,
Ramos F.,
Tortolero M.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04663.x
Subject(s) - azotobacter vinelandii , isolation (microbiology) , characterization (materials science) , microbiology and biotechnology , chemistry , biology , physics , bacteria , genetics , nitrogen fixation , nitrogenase , optics
R‐prime derivatives of plasmid pJB3JI were constructed by conjugal mobilization of Tn5 or Tn5‐Mob induced mutations from Azotobacter vinelandii to Escherichia coli . In A. vinelandii R‐prime formation is mediated by the transposable element IS21. R‐primes resulting from conjugal mobilization of chromosome Tn5‐Mob insertions are very useful for in vivo cloning of markers near the transposon insertion.