Open AccessMethanogenic pathways in Methanosphaera stadtmanae
Author(s) -
Wijngaard W.M.H.,
Creemers J.,
Vogels G.D.,
Drift C.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04662.x
Subject(s) - methanosarcina barkeri , pterin , reductase , chemistry , formaldehyde dehydrogenase , formaldehyde , biochemistry , methanol , enzyme , cofactor , methanogenesis , nad+ kinase , organic chemistry , methane
Methanosphaera stadtmanae reduces methanol to CH 4 in a similar ways as Methanosacina barkeri . Low activities of 5,10‐methylenetetrahydromethanopterin dehydrogenase (MTDH) and reductase (MTR) were found. From studies on formaldehyde oxidation and reduction it was concluded that most likely the inability to reduce CO 2 to CH 4 was due to the lack of an active or the presence of an inactive CO 2 reductase system and methyltetrahydromethanopterin (methyl‐H 4 MPT): coenzyme M methyltransferase. Methanofuran was not detected, while the presence of a pterin, analogous to H 4 MPT, could be substantiated from its degradation products in boiled extracts.