Open AccessCloning and antigenic expression of two genes from Chlamydia psittaci avian strain
Author(s) -
Sato Chiaki,
Katumata Atushi,
Takashima Ikuo,
Hashimoto Nobuo
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04661.x
Subject(s) - chlamydia psittaci , biology , cloning (programming) , strain (injury) , gene , chlamydiaceae , genetics , antigen , chlamydia , microbiology and biotechnology , virology , anatomy , computer science , programming language
Genes from Chlamydia psittaci P‐1041 were cloned into the Bam HI site of pUC19 and were transformed to host Escherichia coli JM109. Two recombinant plasmids that expressed protein antigens of Chlamydia were isolated. The sizes of the DNA fragments were 1350 and 1710 bp, and encoded for polypeptides of M r 25 and 42 kilodaltons (kDa), respectively. The 25‐kDa protein had cross‐reactivity with antisera to ten C. psittaci strains and two C. trachomatis strains, whereas the 42‐kDa protein reacted only with homologous antiserum to the C. psittaci P‐1041 strain. Furthermore, in Southern hybridization analysis these two fragments as probes hybridized with DNA of ten C. psittaci strains and four C. trachomatis strains. These results indicated that the two fragments shared a DNA sequence common to the chlamydial genus.