Open AccessA staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli
Author(s) -
Gadaleta Patricia,
Kaufman Sara,
Martini Patricia,
Zorzópulos Jorge
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04651.x
Subject(s) - amikacin , ampicillin , gentamicin , escherichia coli , plasmid , microbiology and biotechnology , amp resistance , biology , antibiotics , genetics , gene
Abstract: Plasmid pPG1 from Staphylococcus aureus coding for ampicillin (Ap r ), gentamicin (Gm r ) and amikacin (Ak r ) resistance was transformed into Escherichia coli . Transformation efficiency was about 2 × 10 3 transformants/μg of plasmid DNA. The plasmids present in the E. coli transformants were identical to pPG1 according to their restriction patterns. The copy number of pPG1 was estimated to be at least 20‐times less in E. coli than in S. aureus . The minimal inhibitory concentrations (MICs) for Ap and Gm were lower in E. coli than in S. aureus . However, the MIC for Ak was higher in E. coli transformants than in S. aureus . pPG1 was maintained in the E. coli transformants for at least 80 generations at 37°C without antibiotic selection pressure.