Open AccessA novel dye‐linked alcohol dehydrogenase activity present in some Gram‐positive bacteria
Author(s) -
Ophem Peter W.,
Euverink GertJan,
Dijkhuizen Lubbert,
Duine Johannis A.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04636.x
Subject(s) - chemistry , methanol , bacteria , alcohol , alcohol dehydrogenase , biochemistry , ethanol , rhodococcus rhodochrous , formazan , rhodococcus , nuclear chemistry , organic chemistry , enzyme , biology , genetics
Summary An assay was developed which allowed reproducible detection of methanol oxidation by cell free extracts of methanol‐grown Amycolatopsis methanolica . The dye‐linked activity was only observed when high concentrations of phosphate or sulphate salts were applied in the assay. The specific activity strongly increased when raising the amount of cell free extract in the assay. From the large number of electron acceptors tested only the tetrazolium dyes MTT and INT showed significant activity. Activity was observed with primary as well as secondary alcohols. A similar activity was found in ethanol‐grown A. methanolica, Rhodococcus erythropolis and Rhodococcus rhodochrous , but not in other bacteria tested. The identity and possible involvement in alcohol oxidation of this novel dye‐linked activity is discussed.