Open AccessCandida albicans and three other Candida species contain an elongation factor structurally and functionally analogous to elongation factor 3
Author(s) -
Colthurst David R.,
Santos Manuel,
Grant Chris M.,
Tuite Mick F.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04634.x
Subject(s) - elongation , candida albicans , elongation factor , microbiology and biotechnology , eukaryotic translation elongation factor 1 alpha 1 , biology , biochemistry , gene , materials science , ribosome , rna , ultimate tensile strength , metallurgy
Summary A cell‐free poly(U)‐dependent translation elongation system from Candida albicans is ATP‐dependent due to the presence of an elongation factor 3 (EF3)‐like activity. Saccharomyces cerevisiae ribosomes added to a C. albicans post‐ribosomal supernatant (PRS) supported poly(U)‐dependent elongation, suggesting that the C. albicans lysate contained a soluble translation factor functionally analogous to the S. cerevisiae translation factor EF‐3. The presence of EF‐3 in C. albicans was confirmed by Western blotting using an antibody raised against S. cerevisiae EF‐3. This antibody was also used to screen a selection of Candida species, all of which possessed EF‐3 with molecular mass in the range of 110–130 kDa.