Open AccessDetection of heat‐stable enterotoxin in a cholera toxin gene‐positive strain of Vibrio cholerae 01
Author(s) -
Takeda Tae,
Peina Yuan,
Ogawa Akira,
Dohi Sekiko,
Abe Hisao,
Nair G.Balakrish,
Pal S.C.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04630.x
Subject(s) - vibrio cholerae , enterotoxin , vibrionaceae , microbiology and biotechnology , cholera toxin , strain (injury) , toxin , biology , cholera , gene , dna , monoclonal antibody , antibody , bacteria , escherichia coli , genetics , anatomy
Summary DNA colony hybridization with a polynucleotide clonal DNA probe for heat‐stable enterotoxin of Vibrio cholerae non‐01 (NAG‐ST) was used to screen 197 isolates of V. cholerae 01. Under stringent hybridizing and washing conditions, one strain (GP156) reacted with the probe. The concentrated supernatant from V. cholerae 01 GP156, heated at 100°C for 5 min, elicited fluid accumulation in the suckling mice and that could be completely netralized by an anti‐NAG‐ST monoclonal antibody (mAb2F). The preparation from V. cholerae 01 GP156 also inhibited the binding of mAb2F to NAG‐ST in a competitive ELISA. V. cholerae 01 GP156 was confirmed to possess a gene encoding cholera toxin (CT). The results indicate that a heat‐stable enterotoxin is produced by certain strains of CT‐producing V. cholerae 01.