Open AccessUltrastructure of Ureaplasma urealyticum , serotype 8 and the use of immunogold to confirm the localisation of urease and other antigens
Author(s) -
Myles Alison D.,
Russell W.C.,
Davidson I.,
Thirkell D.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04629.x
Subject(s) - immunogold labelling , ureaplasma urealyticum , antigen , ultrastructure , microbiology and biotechnology , ureaplasma , serotype , monoclonal antibody , biology , cytoplasm , electron microscope , mycoplasmataceae , ribonuclease , urease , antibody , mollicutes , enzyme , immunology , biochemistry , mycoplasma , anatomy , physics , gene , optics , rna
Summary Thin sections of Ureaplasma urealyticum were examined by electron microscopy. Most cells were spherical or ovoid, 0.4–0.8 μm in diameter. Utilising previously described monoclonal antibodies and protein Agold, three major antigens were located either on membranes (the 16/17 kDa and 96 kDa antigens) or in the cytoplasm (urease).