Open AccessUse of pulsed field gel electrophoresis to size the chromosome of the bacterial fish pathogen Yersinia ruckeri
Author(s) -
Romalde Jesús L.,
Iteman Isabelle,
Carniel Elisabeth
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04599.x
Subject(s) - yersinia ruckeri , biology , gel electrophoresis , chromosome , pulsed field gel electrophoresis , restriction enzyme , homogeneous , electrophoresis , genetics , pathogen , microbiology and biotechnology , fish <actinopterygii> , dna , physics , gene , fishery , genotype , rainbow trout , thermodynamics
Field inversion gel electrophoresis (FIGE) and contour‐clamped homogenous field (CHEF) electrophoresis were used to analyse the chromosome of Yersinia ruckeri . The 8 base‐pair recognition endonucleases, Not I and Sfi I, generated less than 47 DNA fragments whose size and distribution were appropriate for pulsed field separation. Each isolate displayed a characteristic restriction pattern, with about 20% of bands in common. Depending on the strain used, the estimated genome size for this bacterial fish pathogen ranged from 4460 to 4770 kilobase pairs.