Open AccessVanadate‐resistant mutants of Candida albicans show alterations in phosphate uptake
Author(s) -
Mahanty Sanjoy Kumar,
Khaware Rajkishor,
Ansari Shehnaz,
Gupta Poonam,
Prasad Rajendra
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04590.x
Subject(s) - vanadate , phosphate , candida albicans , mutant , yeast , derepression , biology , corpus albicans , microbiology and biotechnology , biochemistry , chemistry , gene expression , gene , psychological repression
Phosphate uptake studies in different strains of the dimorphic pathogenic yeast Candida albicans were undertaken to show that this yeast actively transported phosphate with an apparent K m in the range of 90–170 μM. The uptake was pH dependent and derepressible under phosphate starvation. Vanadate‐resistant ( van ) mutants of C. albicans showed a 20–70% reduction in the rate of phosphate uptake in high phosphate medium and was associated with an increased K m and reduced V max . The magnitude of depression under phosphate starvation was different between van mutants. These results demonstrate that van mutants may have developed resistance by modifying the rate of entry of vanadate.