Open AccessConfirmation by using mutant strains that the membrane‐bound H + ‐ATPase is the major source of non‐linear dielectricity in Saccharomyces cerevisiae
Author(s) -
Woodward Andrew M.,
Kell Douglas B.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04575.x
Subject(s) - vanadate , harmonics , saccharomyces cerevisiae , mutant , atpase , enzyme , yeast , chemistry , biochemistry , analytical chemistry (journal) , chromatography , physics , voltage , gene , quantum mechanics
Non‐linear dielectric spectroscopy is a novel technique for determining the activity of (predominantly) membranous enzymes as their ability to generate harmonics when excited with a sinusoidal electrical field. In washed suspensions of yeast cells, the ability to generate harmonics is inhibited by low concentrations of sodium vanadate, suggesting that the vanadate‐sensitive H + ‐ATPase is the major source of the non‐linear dielectricity. This conclusion is greatly strengthened by the demonstration herein that the generation of harmonics by a strain containing a vanadate‐resistant H + ‐ATPase is also highly resistant to sodium metavanadate.