Open AccessGrowth, sporulation and enterotoxin production by Clostridium perfringens type A in the presence of human bile salts
Author(s) -
Heredia Norma L.,
Labbe Ronald G.,
Rodriguez Manuel A.,
GarciaAlvarado Jose S.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04561.x
Subject(s) - enterotoxin , clostridium perfringens , microbiology and biotechnology , sodium cholate , sodium , toxin , chemistry , clostridiaceae , growth inhibition , biology , biochemistry , bacteria , in vitro , escherichia coli , genetics , organic chemistry , gene
The effect of human bile juice and bile salts (sodium cholate, sodium taurocholate, sodium glycochenodeoxycholate and sodium chenodeoxycholate) on growth, sporulation and enterotoxin production by enterotoxin‐positive and enterotoxin‐negative strains of Clostridium perfringens was determined. Each bile salt inhibited growth to a different degree. A mixture of bile salts completely inhibited the growth of enterotoxin‐positive strains of this organism. Human bile juice completely inhibited growth of all the strains at a dilution of 1:320. A distinct stimulatory effect of the bile salts on sporulation was observed in the case of C. perfringens strains NCTC 8239 and NCTC 8679. The salts also increased enterotoxin concentrations in the cell extracts of the enterotoxin‐positive strains tested. No effect on enterotoxin production was detected when an enterotoxin‐negative strain was examined.