Open AccessPhysical mapping of the Mycoplasma capricolum genome
Author(s) -
Miyata Makoto,
Wang Lu,
Fukumura Takahashi
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04550.x
Subject(s) - genome , restriction enzyme , biology , genome size , genetics , restriction map , pulsed field gel electrophoresis , gel electrophoresis , microbiology and biotechnology , dna , gene , plasmid , genotype
Summary A physical map of Mycoplasma capricolum ATCC 27343 genome was constructed, based on estimation of the restriction fragment sizes by pulse‐field electrophoresis. The linkage order of restriction fragments was determined by two‐dimensional electrophoresis of partial and complete single digests and complete double digests and by Southern hybridization analysis. The genome size was established at 1155.5 kb, and 26 cleavage sites for 7 endonucleases were assigned to the map.