Open AccessRegulation of biosynthesis of l ‐amino acid oxidase by Neurospora crassa
Author(s) -
Niedermann Denise M.,
Lerch Konrad
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04547.x
Subject(s) - neurospora crassa , biochemistry , oxidase test , cycloheximide , amino acid , crassa , phenylalanine , biology , enzyme , biosynthesis , gene , phosphofructokinase 2 , protein biosynthesis , microbiology and biotechnology , mutant
Summary l ‐Amino acid oxidase was purified from liquid cultures of Neurospora crassa induced by l ‐phenylalanine, d ‐phenylalanine, ATP and cycloheximide. Although the four enzyme species isolated were found to differ in size and electrophoretic mobility, they were the product of a single gene as demonstrated by genomic Southern analysis. Northern analysis of total cellular RNA showed a rapid increase of l ‐amino acid oxidase mRNA in response to the different inducing agents studied. These data suggest that the l ‐amino acid oxidase gene from N. crassa is regulated at the transcriptional level.