Open AccessIn vitro germination of single conidia of the aphid‐pathogenic fungus Erynia neoaphidis and phenotypic variation among sibling strains
Author(s) -
Gray Simon N.,
Wilding Neil,
Markham Paul
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04541.x
Subject(s) - biology , conidium , aphid , germination , infectivity , fungus , in vitro , phenotype , botany , virulence , aphididae , microbiology and biotechnology , genetics , pest analysis , homoptera , gene , virus
Summary A simple method has been developed for triggering the germination of isolated Erynia neoaphidis conidia in vitro. This was used to obtain genetically pure monoconidial isolates of the fungus for the first time in vitro. The phenotypes of such isolated were characterized on the basis of colony radial growth rate, colony peripheral growth zone width, specific growth rate and aphid‐infectivity. Significant differences were demonstrated between isolates from a single parental colony for the first two of these characteristics, suggesting that several different genotypes could be derived from a single field isolate.