Open AccessNon‐radioactive restriction fragment length polymorphism (RFLP) typing of Clostridium difficile
Author(s) -
Bowman Rodney A.,
O'Neill Gael L.,
Riley Thomas V.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04540.x
Subject(s) - restriction fragment length polymorphism , typing , terminal restriction fragment length polymorphism , clostridium difficile , biology , labelling , clostridium , clostridiaceae , ribosomal rna , microbiology and biotechnology , polymerase chain reaction , genetics , bacteria , gene , biochemistry , antibiotics
Summary A typing method for Clostridium difficile based on restriction fragment length polymorphisms (RFLP) is described. The technique utilizes commercially available Escherichia coli ribosomal ribonucleic acid (rRNA) as probe material. Probe labelling, hybridization and detection was performed using the Enhanced Chemiluminescence (ECL) gene detection system. The probe labelling procedure was easy to perform, taking only 20 min. The complete typing method was comparatively simple, reproducible and readily adaptable to most bacterial genera.