Open AccessLow copy number vectors for expression of fused genes to β‐galactosidase in Escherichia coli
Author(s) -
Bingham Alistair H.A.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04535.x
Subject(s) - lac repressor , repressor , plasmid , escherichia coli , beta galactosidase , expression vector , lac operon , gene , biology , vector (molecular biology) , microbiology and biotechnology , gene expression , chemistry , genetics , recombinant dna
Summary A series of six expression vectors, pXM184 Lac.A, B, C, pXM184Z.A, B, C, based on the low copy plasmid pACYC184 that allow for expression of proteins fused to β‐galactosidase in Escherichia coli is described. A level of 50 000 units of β‐galactosidase is routinely observed and is easily identifiable on protein gels. This paper also reports the tight regulation of expression of the Trc promoter in these vectors using the LacI q repressor.