Open AccessVariability in LPS composition, antigenicity and reactogenicity of phase variants of Bordetella pertussis
Author(s) -
Ray Ava,
Redhead Keith,
Selkirk Susanna,
Poole Stephen
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04531.x
Subject(s) - bordetella pertussis , antigenicity , microbiology and biotechnology , lipopolysaccharide , pertactin , phase variation , haemophilus influenzae , pertussis toxin , reactogenicity , biology , chemistry , bacteria , immunology , biochemistry , g protein , immunogenicity , immune system , antibody , receptor , virulence , genetics , gene , antibiotics
Summary Comparison of lipopolysaccharides (LPS) from phase variants of different strains of Bordetella pertussis has shown a difference in their composition, antigenicity and reactogenicity. Phase I variants of B. pertussis , with the exception of strain 134, contain a preponderance of LPS I whereas the major component of LPS of phase IV variants in LPS II. Sera raised to LPSs of phase I strains, other than 134, cross‐react with each other but not with phase IV LPSs; and similarly all sera raised to phase IV LPSs cross‐react with each other and with LPS from 134 phase I. The LPSs of all phase I variants, including that of 134, are approximately ten‐fold or more reactive in the limulus amoebocyte lysate assay (LAL) than phase IV LPSs. In the human mononuclear cell pyrogen assay phase IV LPSs also stimulated a lower response than phase I LPSs. The B. pertussis phase I LPSs are 10‐times more reactive than Escherichia coli standard endotoxin in the LAL assay but 100‐times less reactive than E. coli LPS in the monocyte test for pyrogen. The SDS‐PAGE profiles of B. pertussis LPSs are quite different from those of B. parapertussis and B. bronchiseptica strains. B. pertussis LPSs produced a typical lipooligosaccharide (LOS) pattern. B. bronchiseptica LPS produced a similar pattern but was antigencally from B. pertussis LPSs I and II. B. parapertussis in contrast produced a ladder pattern typical of smooth type LPS.