Open AccessIdentity of hemolysins produced by Bacillus thuringiensis and Bacillus cereus
Author(s) -
Honda Takeshi,
Shiba Atsuko,
Seo Shigeko,
Yamamoto Junko,
Matsuyama Junko,
Miwatani Toshio
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04530.x
Subject(s) - bacillus cereus , hemolysin , bacillus thuringiensis , microbiology and biotechnology , cereus , toxin , hemolysis , bacillales , biology , bacillaceae , sephadex , chemistry , chromatography , biochemistry , bacillus subtilis , bacteria , virulence , immunology , gene , enzyme , genetics
Summary A hemolysin (Bt‐hemolysin) produced by Bacillus thuringiensis var. kurstaki HD‐1 producing crystalline toxin(s) was purified by successive treatments of ammonium sulfate (45–65%) and column chromatography using DEAE‐cellulose, Sephadex G‐75 and KB‐002 (a hydroxyapatite column for fast protein liquid chromatography). A hemolysin (Bc‐hemolysin) produced by B. cereus HG‐6A was also purified by the same procedure. The purified Bt‐hemolysin and Bc‐hemolysin, both of which are thiol‐activated hemolysins, were biologically, physicochemically and immunologically identical. These findings provide further evidence of the similarity of B. thuringiensis , which is being used as a biological insecticide, to B. cereus , a toxigenic organism of food poisoning.