Open AccessTransfer of Tn 916 and Tn 916 ΔE into Clostridium difficile : demonstration of a hot‐spot for these elements in the C. difficile genome
Author(s) -
Mullany Peter,
Wilks Mark,
Tabaqchali Soad
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04527.x
Subject(s) - clostridium difficile , transposable element , microbiology and biotechnology , biology , genome , bacillus subtilis , genetics , bacteria , gene , antibiotics
Summary The conjugative transposon Tn 916 and a derivative Tn 916 ΔE was transferred from Bacillus subtilis into Clostridium difficile CD37 by filter mating. All the C. difficile transconjugants appeared to contain one copy of the transposon integrated into the same position in the genome. Transposition from the original site of integration was not observed. Like Tn 916 the transferable tetracycline resistance determinant (Tc‐CD) of C. difficile has a preferred site of integration in C. difficile and is homologous with Tn 916 along the whole length of Tn 916 . However comparisons of the distribution of Taq I and Sau 3AI sites in the homologous regions of the two elements did not demonstrate any hybridizing fragments in common.