Open AccessDetection of neutral sugars in purified type G botulinum progenitor toxin and the effects of some glycolytic enzymes on its molecular dissociation and oral toxicity
Author(s) -
Nukina Masafumi,
Miyata Tsutomu,
Sakaguchi Sumiko,
Sakaguchi Genji
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04522.x
Subject(s) - toxin , dithiothreitol , toxicity , molecular mass , clostridium botulinum , biochemistry , chemistry , enzyme , sephadex , microbiology and biotechnology , biology , organic chemistry
Summary Arabinose and galactose were detected in purified type G botulinum toxin ( M r about 500 000) of Clostridium argentinense . The i.p. LD 50 /mg N of type G progenitor toxin was one‐tenth, but the oral LD 50 /mg N twice that of type A‐L toxin. The lysozyme‐, endo‐β‐galactosidase‐, and N ‐glucanase‐treated toxins each had a molecular mass of about 300 000. The oral toxicity of the endo‐β‐galactosidase ot N ‐glucanase‐treated toxin was one‐fifth that of untreated progenitor toxin. On DEAE‐Sephadex chromatography, the N ‐glucanase‐treated toxin dissociated into two fractions, nontoxic and toxic. SDS‐PAGE of the toxic fraction showed a single band with a M r of about 1500 000, and after dithiothreitol treatment, two bands with M r of 100 000 and 50 000.