Open AccessInvolvement of the collagenase produced by Corynebacterium rathayii in its adhesion to collagen
Author(s) -
Labadie J.C.,
Gaillard B.,
Potier P.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04508.x
Subject(s) - collagenase , adhesion , bacteria , chemistry , microbiology and biotechnology , microbial collagenase , interstitial collagenase , biochemistry , biology , enzyme , organic chemistry , genetics
Summary The bacterium previously called Empedobacter collagenolyticum which has been recently reclassified as C. rathayii , adheres to collagen. The mechanism of this binding was analyzed by incubating the microorganism with substrates which induced or inhibited the collagenase synthesis. Immunoblots of bacterial cells able to adhere to collagen showed that collagenases are always detected and treatment of the bacterium with antibodies raised against purified collagenase inhibited their adhesion. Hydrophobic interactions between the collagenase and collagen also influence the adhesion. The results suggest that collagenase plays a major role in the adhesion of the bacterium to collagen fibres.