Open AccessMolecular cloning of Renibacterium salmoninarum DNA fragments
Author(s) -
Etchegaray Jean Pierre,
Martínez Maria Adela,
Krauskopf Manuel,
León Gloria
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04505.x
Subject(s) - biology , molecular cloning , recombinant dna , dna , microbiology and biotechnology , gene , cloning (programming) , dna sequencing , hybridization probe , genetics , peptide sequence , computer science , programming language
Summary A Renibacterium salmoninarum enriched recombinant DNA library was constructed to isolate DNA fragments which could be used as probes to detect gene sequences specific for the causative agent of bacterial kidney disease in salmonid fish. One fragment of 149 base pairs was isolated and its specificity and sequence determined. This probe may prove useful in the design of diagnostic tests for the disease in asymptomatic fish and ova.