Open AccessCloning and characterization of a DNA fragment that complements the nfxB mutation in Pseudomonas aeruginosa PAO
Author(s) -
Okazaki Takashi,
Iyobe Shizuko,
Hashimoto Hajime,
Hirai Keiji
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04499.x
Subject(s) - pseudomonas aeruginosa , plasmid , transformation (genetics) , mutant , biology , dna , microbiology and biotechnology , mutation , aminoglycoside , molecular cloning , cloning (programming) , genetics , bacteria , gene , antibiotics , peptide sequence , computer science , programming language
Summary The 2.2‐kilobase DNA fragment that can complement the nfxB mutation in Pseudomonas aeruginosa PAO was cloned from chromosomal DNA of P. aeruginosa PAO1. The nfxB mutants show an increase in resistance to quinolones and hypersusceptibility to β‐lactam and aminoglycoside antibiotics. In the mutants, a 54 kDa outer membrane protein newly appeared. The phenotype was restored to the wild‐type by transformation with plasmid carrying the 2.2‐kb DNA fragment.