Open AccessAnalysis of the antigenic difference between Vero toxin 2 (VT2) and VT2 variant (VT2vh) of Verotoxin‐producing Escherichia coli by a site‐directed mutagenesis
Author(s) -
Ito Hideaki,
Nishibuchi Mitsuaki,
Takeda Yoshifumi
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04498.x
Subject(s) - antigenicity , biology , mutagenesis , toxin , vero cell , escherichia coli , protein subunit , microbiology and biotechnology , ouchterlony double immunodiffusion , mutant , site directed mutagenesis , antigen , biochemistry , gene , genetics , in vitro , antiserum
Summary Ouchterlony double gel diffusion analysis of the A and B subunits of purified Vero toxin 2 (VT2) and a variant of VT2 (VT2vh) demonstrated that the difference in antigenicity between VT2 and VT2vh is due to the difference in the B subunit of the two toxins. Analysis of mutants of VT2vh prepared by site‐directed mutagenesis attributed the antigenic dissimilarity to the difference in the amino acid residue at position 24 of the B subunit.