Open AccessAn insertion of Escherichia coli transposable element IS 1K into the site immediately before tetracycline‐resistance determinant of Bacillus subtilis chromosomal DNA fragment in cloning in E. coli
Author(s) -
Amano Hitoshi,
Sakaguchi Reiko,
Shishido Kazuo
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04494.x
Subject(s) - cloning (programming) , tetracycline , fragment (logic) , dna , genetics , escherichia coli , biology , molecular cloning , microbiology and biotechnology , chemistry , gene , antibiotics , peptide sequence , computer science , programming language
Summary In cloning in Escherichia coli C600 of a 4.5‐kbp Hin dIII DNA fragment with the tetracycline‐resistance determinant ( tet BS908) from Bacillus subtilis GSY908 chromosome using a plasmid vector, a 5.2‐kbp Hin dIII DNA fragment was also isolated at a ratio of 2 to 89. The two independently obtained 5.2‐kbp fragments were an insertion derivative of the 4.5‐kbp fragment and carried E. coli transposable element IS 1K , which was inserted at the same site immediately before tet BS908 in the same direction. For the IS 1K insertions, the 8‐bp sequence CAAATTTT was used as a target, this having no similarity to any published sequences.