Open AccessCharacterization of the assimilatory and dissimilatory nitrate‐reducing systems in Rhodobacter : a comparative study
Author(s) -
MartínezLuque Manuel,
Dobao M.M.,
Castillo F.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04485.x
Subject(s) - rhodobacter , nitrate reductase , nitrite reductase , nitrate , rhodospirillaceae , rhodobacter sphaeroides , chemistry , biochemistry , nitrite , periplasmic space , cytosol , reductase , rhodospirillales , enzyme , photosynthesis , mutant , organic chemistry , escherichia coli , gene
Assimilatory nitrate reductase from Rhodobacter capsulatus E1F1 was found to be a cytosolic NADH‐dependent enzyme whereas nitrite reductase was detected both in the periplasm and in the cytosol. In addition to nitrate and nitrite, organic nitro compounds were also able to induce nitrate reductase which was repressed in carbon‐starved cells or under conditions producing low intracellular nitrate levels. By contrast, nitrate reductase from Rhodobacter sphaeroides DSM 158 was located in the periplasm, did not use NADH as electron donor in vitro and was induced independently from the carbon/nitrogen balance of the cells. Production of nitrogenous gases was never detected in either strain cultured anaerobically with nitrate or nitrite.