Open AccessMultiple xylanases of Cellulomona fimi are encoded by distinct genes
Author(s) -
Clarke Jonathan H.,
Laurie Judith I.,
Gilbert Harry J.,
Hazlewood Geoffrey P.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04481.x
Subject(s) - xylanase , cellulase , genomic dna , escherichia coli , gene , restriction enzyme , dna , biology , restriction map , genetics , enzyme , biochemistry , base sequence
Cellulomonas fimi genomic DNA encoding xylanase activity has been cloned and expressed in Escherichia coli . As judged by DNA hybridization and restriction analysis, twelve xylanase‐positive clones carried a minimum of four different xylanase ( xyn ) genes. The encoded enzymes were devoid of cellulase activity but three of the four bound to Avicel.