Open AccessUse of a lacZ fusion to study transcriptional regulation of the Rhodobacter capsulatus hemA gene
Author(s) -
Wright Maureen S.,
Eckert John J.,
Biel Susan W.,
Biel Alan J.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04466.x
Subject(s) - rhodobacter , lac operon , gene , transcriptional regulation , fusion gene , regulation of gene expression , chemistry , biology , genetics , gene expression , mutant
Summary An Eco RI fragment containing the Rhodobacter capsulatus hamA promoter has been cloned into a lacZ translational fusion vector. The resulting plasmid produced a hemA‐lacZ fusion protein with a molecular mass of 147 000. Expression of the hemA‐lacZ fusion, as measured by production of β‐galactosidase, was regulated 2‐ to 3‐fold by oxygen tension. The unexpectedly small change in β‐galactosidase levels suggests that transcriptional regulation of the hemA gene is not the major factor in oxygen‐mediated control of porphyrin synthesis.