Open AccessCloning and partial characterization of genes for ribosomal ribonucleic acid in Lactococcus lactis subsp. lactis
Author(s) -
Beresford T.,
Condon S.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04463.x
Subject(s) - cosmid , biology , gene , lactococcus lactis , ribosomal rna , restriction enzyme , genetics , 16s ribosomal rna , 23s ribosomal rna , microbiology and biotechnology , restriction map , nucleic acid sequence , rna , bacteria , ribosome , lactic acid
Summary A cosmid gene library of the genome of Lactococcus lactis subsp. lactis 712 was probed for the presence of 16S rRNA genes, using 32 P 5′ end‐labelled 16S rRNA fragments. Cosmid DNA from positive clones responsible for hybridisation was subcloned into a high copy number vector and a restriction map was constructed. The location of the 16S, 23S and 5S rRNA genes was determined on this map. Transcriptional promoter activity was identified upstream of the 5′ end of the 16S rRNA gene. By probing L. lactis 712 chromosomal DNA cut with a range of restriction endonucleases, with a conserved oligonucleotide to the 5′ end of the 16S rRNA gene, 6 copies of rRNA genes were identified.