Open AccessPseudomonas aeruginosa isolate typing by esterase electrophoresis
Author(s) -
Goullet Ph.,
Picard B.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04442.x
Subject(s) - typing , esterase , serotype , pseudomonas aeruginosa , microbiology and biotechnology , biology , pseudomonadaceae , strain (injury) , enzyme , bacteria , genetics , biochemistry , anatomy
Summary Esterase electrophoretic typing was used to classify clinical isolates of Pseudomonas aeruginosa . One hundred and twenty‐seven P. aeruginosa strains belonging to 16 serotypes (including 16 non‐typeable strains) and isolated from diverse human infections in three hospitals, and the type strain ATCC 10 145, were tested. Four main kinds of esterase and 4 additional esterases were distinguished by their spectra of hydrolytic activity toward synthetic substrates and by their sensitivity or resistance to di‐isopropyl fluorophosphate. The electrophoretic variations of these enzymes were used to define 42 zymotypes. Electrophoretic typing of esterase appeared to be more sensitive than serotyping and the results of the two methods did not correlate. When the two typing methods were used in parallel, 78 different combinations of serotype and zymotype were obtained.