Open AccessThe class 3 outer membrane protein (PorB) of Neisseria meningitidis : gene sequence and homology to the gonococcal porin PIA
Author(s) -
Wolff Karin,
Stern Anne
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04437.x-i1
Subject(s) - porin , bacterial outer membrane , biology , nucleic acid sequence , neisseria meningitidis , homology (biology) , peptide sequence , gene , microbiology and biotechnology , genetics , neisseria , escherichia coli , bacteria
The class 3 protein (PorB) is an important component of the meningococcal outer membrane. The structural gene ( porB ) encoding the class 3 protein has been cloned using primers suitable for the amplification of the corresponding chromosomal fragment by the polymerase chain reaction (PCR). The complete nucleotide sequence was determined and predicts a mature protein of 310 amino acids, preceded by a signal peptide of 19 residues. The predicted protein sequence of the class 3 protein exhibits essential structural homology to the gonococcal porin PIA. The class 3 protein encoding gene was expressed in Escherichia coli under the control of an inducible promotor.