
Recognition sequence of a new methyl‐specific restriction system from Clostridium acetobutylicum strain ABKn8
Author(s) -
Azeddoug H.,
Reysset G.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04435.x
Subject(s) - clostridium acetobutylicum , isoschizomer , restriction enzyme , strain (injury) , clostridium , recognition sequence , biology , sequence (biology) , genetics , microbiology and biotechnology , chemistry , dna , gene , biochemistry , bacteria , anatomy , butanol , gene expression , ethanol , dna methylation
Summary A new type II restriction endonuclease, named Cac II was detected in Clostridium acetobutylicum strain ABKn8. Cac II cleaved the hexanucleotide sequence [5′‐GCN ⇓ NGC‐3′] and generated blunt ends. Up to now no isoschizomer of Cac II has been described.