Open AccessExpression of nrdA and nrdB genes of Escherichia coli is decreased under anaerobiosis
Author(s) -
Casado Cristina,
Llagostera Montserrat,
Barbé Jordi
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04432.x-i1
Subject(s) - escherichia coli , gene , biology , transcription (linguistics) , plasmid , promoter , lac operon , microbiology and biotechnology , gene expression , transcription factor , biochemistry , philosophy , linguistics
By using plasmid nrdA‐lacZ, nrdAB‐lacZ , and nrdB‐lacZ gene fusions, the expression of nrdA and nrdB genes of Escherichia coli under anaerobiosis has been studied. The results obtained show that cells of E. coli growing under either fermentative or nitrate respiring conditions present a lower basal level of both nrdA and nrdB genes transcription from the nrdP A promoter. On the other hand, transcription of the nrdB gene from the internal nrdP B promoter was not affected by the absence of oxygen. Moreover, the DNA damage‐mediated inducing factor of these nrd genes was the same in both aerobic and anaerobic cultures.