Open AccessCharacterization of three soluble c ‐type cytochromes isolated from soybean root nodule bacteroids of Bradyrhizobium japonicum strain CC705
Author(s) -
Appleby Cyril A.,
James Peter,
Hennecke Hauke
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04430.x-i1
Subject(s) - bradyrhizobium japonicum , strain (injury) , cytochrome , bradyrhizobium , cytochrome c , rhizobiaceae , root nodule , biochemistry , biology , chemistry , bacteria , nitrogen fixation , rhizobium , symbiosis , gene , mitochondrion , enzyme , genetics , anatomy
Three soluble, low molecular mass cytochromes c ( M r 8000–15 000) were isolated and purified from soybean root nodule bacteroids of Bradyrhizobium japonicum strain CC705. On the basis of their α: absorbance peaks in the reduced forms, they were named cytochromes c 550 , c 552 and c 555 . Cytochrome c 552 reacted very fast, c 555 very slowly and c 550 not at all with carbon monoxide. The complete amino acid sequence (73 residues) of cytochrome c 552 was established which identifies it as a monoheme, class I cytochrome c with some remote similarity to the cytochrome c 6 family.