Open AccessThe osmotic responses of Saccharomyces cerevisiae in K + ‐depleted medium
Author(s) -
Meikle Amanda J.,
Reed Robert H.,
Gadd Geoffrey M.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04423.x
Subject(s) - glycerol , saccharomyces cerevisiae , vacuole , biochemistry , yeast , dehydrogenase , in vivo , enzyme , growth medium , chemistry , biology , cytoplasm , bacteria , microbiology and biotechnology , genetics
Summary Saccharomyces cerevisiae showed reduced growth in a K + ‐depleted medium but was still capable of synthesizing and accumulating glycerol in the presence of 2.28% (w/v) NaCl; glycerol levels were similar in both K + ‐replete and K + ‐depleted media. The activity of glycerol‐3‐phosphate dehydrogenase, a key enzyme of glycerol synthesis, was inhibited to a similar extent by exogenous K + and Na + although such effects may be modified in vivo due to ionic compartmentation within the vacuole. These results indicate that exogenous K + does not play a significant role in the initiation of glycerol synthesis in osmotically‐stressed yeast.