Open AccessPhosphatase production by a Citrobacter sp. growing in batch cultures retarded by anaerobic or osmotic stress, and the effect of the osmoprotectant glycine betaine
Author(s) -
Hallett D.S.,
Clark P.,
Macaskie L.E.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04407.x
Subject(s) - betaine , osmoprotectant , phosphatase , glycine , biochemistry , alkaline phosphatase , citrobacter , biology , acid phosphatase , osmotic shock , chemistry , enzyme , enterobacteriaceae , escherichia coli , proline , amino acid , gene
Summary The effect of 500 nM NaCl on the growth, and phosphatase production of a Citrobacter sp. was investigated. Although growth was retarded, phosphatase production was enhanced by 50%. Relief from osmotic stress using the osmoprotectant glycine betaine gave normal growth, but phosphatase activity was reduced. The Citrobacter sp. ceased to grow following a shift to anaerobic conditions, but anaerobically‐incubated cells continued to produce phosphatase after a transient lag.