Open AccessCloning and nucleotide sequencing of the gene encoding the β‐lactamase from Citrobacter diversus
Author(s) -
Perilli Mariagrazia,
Franceschini Nicola,
Segatore Bernardetta,
Amicosante Gianfranco,
Oratore Arduino,
Duez Colette,
Joris Bernard,
Frère JeanMarie
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04393.x
Subject(s) - citrobacter , klebsiella oxytoca , nucleic acid sequence , gene , citrobacter freundii , peptide sequence , biology , cloning (programming) , coding region , amino acid , genetics , biochemistry , signal peptide , enterobacteriaceae , escherichia coli , computer science , programming language
The gene coding for the class A β‐lactamase of Citrobacter diversus has been cloned and sequenced. It contains the information for a 294‐amino‐acid precursor protein, including a 27‐residue N ‐terminal signal peptide. The deduced sequence of the N ‐terminal portion of the mature protein is in excellent agreement with that determined by microsequencing of the protein and readily explains the p I differences observed between the naturally occurring forms I and II of the enzyme. The sequence of the mature protein exhibits a very high degree of similarity with that of the Klebsiella oxytoca class A β‐lactamase.