Open AccessComplementation of Methylobacterium organophilum mutants affected in pyrroloquinoline quinone biosynthesis genes pqqE and pqqF by cloned Escherichia coli chromosomal DNA
Author(s) -
Turlin Evelyne,
Biville Francis,
Gasser Francis
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04389.x
Subject(s) - complementation , mutant , escherichia coli , pyrroloquinoline quinone , plasmid , microbiology and biotechnology , dna , biology , biosynthesis , gene , chemistry , biochemistry , enzyme , cofactor
The hybrid plasmid pBGT3, a derivative of pLA2917 containing a 7.8‐kb fragment of Escherichia coli DNA, was found to complement pqqE and pqqF mutants of Methylobacterium organophilum , both impaired in PQQ biosynthesis. The cloned fragment of E. coli DNA did not hybridize with DNA fragments containing pqqE or pqqF previously cloned from M. organophilum . Yet, in M. organophilum mutants, expression of pqqE and pqqF genes from E. coli resulted in a PQQ production estimated at 9–16% of the production observed in M. organophilum wild‐type. The growth rate in methanol medium of the complemented M. organophilum mutants was about 60% of that of the wild‐type.