Open AccessThe role of the O antigen in adjuvant activity of lipopolysaccharide
Author(s) -
Nurminen Marjatta,
Ölander RoseMarie
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04387.x
Subject(s) - lipopolysaccharide , lipid a , toxoid , adjuvant , diphtheria , diphtheria toxin , bordetella pertussis , microbiology and biotechnology , tetanus , antigen , antibody , serotype , salmonella , immunology , salmonella enterica , medicine , immunization , vaccination , biology , toxin , bacteria , genetics
Adjuvant activities of isogenic Salmonella enterica , serovar Typhimurium, O‐6,7 and O‐4,5,12 lipopolysaccharide (LPS), lipid A and Bordetella pertussis LPS were compared by immunizing groups of mice subcutaneously with diphtheria and tetanus toxoid vaccine alone or mixed with one of the LPS derivatives. Five weeks later the mice were bled and the tetanus and diphtheria antibodies in the sera were measured. All the LPS derivatives efficiently increased the antibody responses when compared to the vaccine alone, but the mannose‐rich O‐6,7 LPS and lipid A were significantly more potent than O‐4,5,12 LPS and B. pertussis LPS. We conclude that the quality of the O antigen influences the adjuvant activity of LPS.